Dawn Greving
Department of Biology
Marquette University Faculty Mentor:
Dr. Micheal Schlappi

 My research began with testing several dffferent lines of Arabidopsis thaliana plants to locate a transposon.  It was known that the transposon had jumped to a different region in the genome and testing could show if the transposon was affecting the phenotype of the plant.  Inverse polymerase chain reaction tests were done with restriction enzymes BamHI, EcoRI, EcoRV, and Xhol.  A 250 base pair fragment on the C16 line was found on the 5' end.  An attempt to clone the fragment was tried, but due to many factors this experiment is being redone and will be continued into the fall.In the process of working with different lines of Arabidopsis and running PCR tests it was found that seeds that were thought to be a specific line from a stock center were not the correct line.  The lines of Nd and NO-O were the lines of concern.  Several different primers and control lines were used to confirm that what we thought was's'( was really NO-O and vise versa.  New seeds directly from the stock center were then grown up and flowering times were measured.  The pre data confirms the results that the lines were switched and that the NO-O is and earlier flowering plant than the Nd.The accomplishments of this summers work include locating a fragment that may hold a transposon responsible for a change in the plants phenotype.  Also, finding the difference between the NO-O and Nd Enes of seeds and confirming the fines had been switched.  By carrying both of these projects into the fall more exact data can support my previous accomplishments.